Knowledge-enriched characterization of cell populations. Morphological information from photos can include on the richness of flow cytometry details. As an example, the determine displays an or else standard apoptosis assay applying Annexin V and PI, adding cell imaging to characterize cells in Just about every population to expose morphologically unique characteristics. These insights couldn't are already obtained from multiplex staining by yourself.

Irrespective of whether you’re a starter or an expert, examine our assets to progress your investigate and expertise in flow cytometry.

Combine acoustic concentrating technologies with superior-parameter Assessment for versatile spectral unmixing and standard workflows.

The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating having a large-speed brightfield camera for simultaneous significant throughput flow cytometry and high resolution brightfield imaging.

*Pricing is indicative only and it is subject matter to vary. You should Call your Agilent representative with any queries. The information established forth herein isn't an offer or a motivation, each of that are subject to credit history approval, terms and conditions, and availability with Agilent. Agilent Monetary Remedies is accessible in chosen countries.

In this article, a highly trained consumer has gated singlets confidently. Following evaluating the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of greater than four% aggregates.

Pair your Attune flow cytometer having an autosampler for greater workflow efficiency, and insert robotic integration for optimum throughput.

Our researchers will style panels for yourself as repeatedly you'll need—gratis, no solution obtain necessary.

Early indications of this contamination experienced at first been dismissed as particles. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow read more Cytometer also enables you to use morphological capabilities to find out attention-grabbing subpopulations that could not be apparent from flow cytometry details alone.

Probably most of all, these activities incorporate cells of Evidently distinct phenotypes which could lead on to incorrect conclusions with regards to double optimistic situations (especially in exceptional populations).

AI-pushed automated graphic analysis Along with the Attune Cytometric Software translates celebration features into distinct morphology parameters that could be coupled with regular fluorescence and scatter parameters to expose really reproducible populace changes and exclusive subsets.

We Beforehand demonstrated the power of imaging Motor vehicle-T/Ramos mobile interactions. Permit’s look at just the inhabitants of greatest curiosity, the double favourable occasions, to learn more. We are able to now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to even more analyze the characteristics of such populations and refine gating on these events, growing knowledge robustness.

Built-in dimensionality reduction resources greater resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify envisioned population resolution in lysed full human blood. User can check out functions from Each and every cluster for visual confirmation of cell phenotypes.

No wash/no lyse leukocyte Evaluation with imaging. Cells have been obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Specialist UX/UI allows consumers to coach AI versions for expanded applications. (A) The “Practice Model” characteristic delivers annotation tools and genuine-time product coaching precision.

At the guts of each Attune Flow Cytometer lies our revolutionary acoustic concentrating technological know-how, which utilizes audio waves to meticulously align cells throughout the flow cell. This impressive strategy surpasses classic hydrodynamic techniques, leading to more quickly processing speeds, Outstanding knowledge high quality, and elevated sample throughput.

As the mobile line experienced Earlier been analyzed to the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and were capable of document the microbial an infection at the least 5 days earlier.

The Attune CytPix Flow Cytometer makes it possible for customers to visualise samples in the course of acquisition, validate the accuracy of gating procedures, and aid effects with responsible documentation for publication uses.

What appeared to be a homogeneous inhabitants is actually a lot more assorted—an insight that should be regarded as when interpreting success.